Soluble vs. Insoluble Enzyme Substrates Soluble vs. Insoluble Enzyme Substrates Selecting the right enzyme substrate can make or break your assay. As we explored in Part 1, enzyme substrates are essential for detecting enzymatic activity. But should you use a soluble or insoluble substrate? The choice depends on your assay’s goals: should you prioritize quantitative
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Advancing E. coli Detection with Chromogenic and Fluorogenic Substrates Advancing E. coli Detection with Chromogenic and Fluorogenic SubstratesEscherichia coli (E. coli) detection has evolved far beyond traditional culture and PCR methods. While enzyme substrates like β-D-glucuronidase (GUS) and β-D-galactosidase (GAL) have long been used to target E. coli’s unique biomarkers, next-generation substrates such as Aldol®, RUG®, and AquaSpark® are now overcoming the
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